DNA enzymes: New-found chemical reactivity
نویسنده
چکیده
DNA is generally considered to be chemically rather inert, but self-cleaving DNA molecules have recently been isolated from in vitro selection experiments; their properties advance the idea that, like RNA, DNA can serve as both a catalyst and an information storage medium.
منابع مشابه
The Prediction of Thermo Physical, Vibrational Spectroscopy, Chemical Reactivity, Biological Properties of Morpholinium Borate, Phosphate, Chloride and Bromide Ionic Liquid: A DFT Study
In the light of computational chemistry, based on morpholinium cation-based Ionic Liquid, their different types of physical, chemical, and biological properties is highlighted. The physical properties are evaluated through the Density Functional Theory (DFT) of Molecular Mechanics and also examine the chemical and biological properties. The difference between Highest Occupied Molecular Orbital ...
متن کاملDiethyl pyrocarbonate: a chemical probe for DNA cruciforms.
Two palindromic DNA sequences were analyzed with respect to their chemical reactivities with diethyl pyrocarbonate. In negatively supercoiled plasmid templates enhanced N7 carbethoxylation was found with individual purines located in presumptive single-stranded loops of DNA cruciform structures. No enhanced reactivity at these positions was observed in linear, relaxed or low superhelical densit...
متن کاملElucidating Organ-Specific Metabolic Toxicity Chemistry from Electrochemiluminescent Enzyme/DNA Arrays and Bioreactor Bead-LC-MS/MS.
Human toxic responses are very often related to metabolism. Liver metabolism is traditionally studied, but other organs also convert chemicals and drugs to reactive metabolites leading to toxicity. When DNA damage is found, the effects are termed genotoxic. Here we describe a comprehensive new approach to evaluate chemical genotoxicity pathways from metabolites formed in-situ by a broad spectru...
متن کاملOrdered Multistep Synthesis in a Single Solution Directed by DNA TemplatesFunding was generously provided by the NIH/NIGMS (R01[thinsp]M065865), the Office of Naval Research (N00014-03-1-0749), and an NSF Graduate Research Fellowship to T.M.S
The use of DNA templates to direct chemical reactions enables the principles of biological evolution to be applied to the discovery of synthetic molecules and new chemical reactions. In addition, the use of DNA hybridization to modulate the effective molarity of DNA-linked reactants allows synthetic molecules to be manipulated in ways that cannot be accessed using traditional synthetic methods....
متن کاملDifferential minor groove interactions between DNA polymerase and sugar backbone of primer and template strands.
DNA polymerases are the key enzymes for DNA synthesis involved in DNA replication, recombination, and repair. These enzymes undergo manifold contacts with the primer-template-substrates reaching up to several nucleotide pairs beyond the catalytic centre. To evaluate these enzyme contacts with the DNA substrates we applied novel synthetic steric probes in functional studies. We found that throug...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Current Biology
دوره 7 شماره
صفحات -
تاریخ انتشار 1997